Product Info Summary
SKU: | M12477-9 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | IF, ICC, WB |
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Product info
Product Name
Anti-Histone H3 (mono+di+tri methyl K14) HIST1H3A Rabbit Monoclonal Antibody
SKU/Catalog Number
M12477-9
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Histone H3 (mono+di+tri methyl K14) HIST1H3A Rabbit Monoclonal Antibody catalog # M12477-9. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Histone H3 (mono+di+tri methyl K14) HIST1H3A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M12477-9)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
I-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Histone H3 (mono+di+tri methyl K14)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M12477-9 is reactive to in Human, Mouse
Applications
M12477-9 is guaranteed for IF, ICC, WB Boster Guarantee
Observed Molecular Weight
17 kDa
Calculated molecular weight
15404 MW
Background of
Putative transcription factor involved in pancreas development and function.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Histone H3 (mono+di+tri methyl K14) expression in HeLa cell lysate.
Specific Publications For Anti-Histone H3 (mono+di+tri methyl K14) HIST1H3A Rabbit Monoclonal Antibody (M12477-9)
Hello CJ!
M12477-9 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Role of endoplasmic reticulum stress in apoptosis of differentiated mouse podocytes induced by high glucose
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-Histone H3 (mono+di+tri methyl K14) HIST1H3A Rabbit Monoclonal Antibody
Question
My lab would like using your anti-Histone H3 (mono+di+tri methyl K14) Rabbit Monoclonal antibody for hdacs deacetylate histones studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-09-18
Answer
We appreciate your inquiry. This M12477-9 anti-Histone H3 (mono+di+tri methyl K14) Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for IF, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-09-18